The activity of this multi-site platform is organized around 3 major areas of expertise:
- Imaging: morphometric analyses, macroscopy, confocal microscopy, spectral microscopy, multiphoton microscopy, long-term time-lapse microscopy, whole-slide imaging, morphological & functional echography.
- Flow Cytometry: cellular and molecular analyses, spectral analyses, imaging flow cytometry.
- High-speed cell sorting: cell sorting and cloning.
EQUIPMENTS
Small animal imaging
- Microendoscope confocal fibré Cellvizio Dual Band Lab (Mauna Kea Technologies).
- Échographe Vevo 770 (Visual Sonic).
- IVIS Xenogen (Perkin Elmer).
Photonic imaging
- Leica TCS SP8 STED 3X super-resolution microscope.
- LSM880 – AiryScan (Zeiss) high-resolution spectral-imaging confocal microscope outfitted for the study of living organisms.
- Total internal reflection florescence microscope (TIRFm) equipped for FRET microscopy.
- Digital slide scanner AxioScan (Zeiss).
- 3 Microvision fluorescence-mode morphometric workstations on upright/inverted microscopes.
- IncuCyte Zoom system (Essen Bioscience), an intra-incubator time-lapse cell culture imaging system.
- Biostation IM (Nikon).
- Automated fluorescence video-microscope.
Flow cytometry: cell analysis and sorting (BSL2 lab environment)
- Two ASTRIOS-EQ high-speed cell sorters (Beckman Coulter).
- SH800 (Sony) cell sorter / cloner equipped with single-use microfluidics sorting chips.
- SP6800 spectral analyzer (Sony).
- ImageStreamX imaging flow cytometer (Amnis).
- LSR II analyzer (Becton Dickinson).
- CytoFlex analyzer (Beckman Coulter).
- Two MACSQuant 10 (Miltenyi Biotec) analyzers.
- C6 cytometer (BD Accuri). FC 500 MCL analyzer (Beckman Coulter).
- Luminex BioPlex 200 cytometer (Biorad).
- Multisizer 4 (Beckman Coulter).
Analysis
Metamorph, Imaris, Fiji, ImageJ, FlowJo, Kaluza, and other software.