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As France's leading biocluster, Genopole is an incubator for cutting-edge projects in biotechnology. Located in the city of Évry, just south of Paris, Genopole provides a unique environment for scientists and entrepreneurs seeking to advance research and innovation.

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Advantages

Genopole accompanies researchers, postdocs and start-up entrepreneurs through all the phases of their projects to ensure the best possible conditions for business development.

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Genopole’s citizens

Every day, at Genopole, researchers, entrepreneurs and students cross paths, share ideas and unite forces in a veritable melting pot for innovation.

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Highlights

Giving wings to research and empowering employment in our community are cornerstones of Genopole's mission. Catch up on recent scientific advances, the accomplishments of our biotech actors and the events that enliven the biocluster.

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Platforms

Electron Microscopy Platform


Ecole des Mines de Paris Centre des Matériaux BP 87
91003 EVRY cedex FRANCE
Phone : +33 1 60 76 31 73
Email : mohamed.sennour@mines-paristech.fr

Technical Manager : SENNOUR Mohamed
Web Site (french) >
view of cell in electronic microscopy view of cell in electronic microscopy

Host:

Centre des Matériaux Pierre Marie Fourt de l’Ecole des Mines de Paris

#Technological Platform #Cell Biology – Microscopy

  • Electron microscopy
  • Imaging
  • Tomography
  • Ultramicrotomy
  • Ultrastructure
  • Cellular biology

Supervisors :

ENSMP, Mines ParisTech

Field of activity


Platform dedicated to electron microscopy in the fields of cell and tissue biology and material physics

Cell and tissue biology :

  • Ultrastructural morphology
  • Ultrastructural immunocytochemistry
  • Nanoparticles for protein targeting.

Material physics :

  • Nanomaterials and new alloys
  • Interfaces: structural, damage and properties
  • Protection of materials, multimaterials.

EQUIPMENTS

Transmission electron microscopy (FEI Tecnai F20)

  • Schottky field emission gun; acceleration voltage: 80–200 kV; resolution: 0.24 nm
  • specimen stage angle: ±30° (80° in nanotomographic mode)
  • Imaging in conventional and high-resolution modes with a magnification ranging from x50 to x1,000,000 (20 million on the CCD camera)
  • Scanning transmission electron microscopy (STEM) with bright-field detector (BF), annular dark field (ADF) and high-angle annular dark field (HAADF) modes
  • Local chemical analysis with an energy-dispersive X-ray spectrometer coupled to a nanometer probe
  • Energy-filtered imaging system (GIF) coupled to an electron energy loss spectrometer (EELS).

Scanning electron microscopy (FEI Nova NanoSEM 450)

  • Schottky field emission gun; beam acceleration voltage: 50 V – 30 KV; resolutions: 1 nm at 15 kV and 1.4 nm at 1 kV. High-resolution column with sufficient current for rapid EDS mappings and EBSD analyses at low and high magnification
  • Low Vacuum” mode for the observation of non-conductive specimens
  • Secondary electron (SE) or backscatter electron (BSE) imaging studies using in-chamber or in-lens detectors with a useable magnification range from x35 to 600,000 (field width between 3.6 mm and 200 nm). Automated multi-field acquisition
  • Tunable-energy X-ray microanalysis with point, multi-point and mapping modes and qualitative and quantitative assessment
  • EBSD rapid acquisition system for crystal orientation mapping.

Samples preparation :

  • Ultramicrotomy: LEICA EMTRIM and LEICA ULTRACUTR
  • Plasma cleaner
  • Tint: Lynx and Microscopy Tissue Process
  • Sputter coater: Cressington 208HR
  • Carbon coater: Quorum Q150TE

ACCESS:

Contact : Julien Picot
Phone: +33 1 60 87 35 15 – email: julien.picot@genopole.fr

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#Cell Biology


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